Introduction of selective intersubunit disulfide bonds into self-assembly protein scaffold to enhance an artificial multienzyme complex's activity.
Hirakawa H, Kakitani A, Nagamune T
Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. Epub 2013 Feb 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66139 | pET15b+PCNA1 WT | Expresses the wild type of Sulfolobus solfataricus PCNA1 |
| 66140 | pET15b+PCNA1 G108C | Expresses the G108C mutant of Sulfolobus solfataricus PCNA1 |
| 66173 | pET15b+PCNA1 P186C | Expresses the P186C mutant of Sulfolobus solfataricus PCNA1 |
| 66174 | pET15b+PCNA1 G108C/P186C | Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1 |
| 66175 | pET15b+PCNA2 WT | Expresses the wild type of Sulfolobus solfataricus PCNA2 |
| 66176 | pET15b+PCNA2 E105C | Expresses the E105C mutant of Sulfolobus solfataricus PCNA2 |
| 66177 | pET15b+PCNA2 L171C | Expresses the L171C mutant of Sulfolobus solfataricus PCNA2 |
| 66178 | pET15b+PCNA2 E105C/L171C | Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2 |
| 66179 | pET15b+PCNA3 WT | Expresses the wild type of Sulfolobus solfataricus PCNA3 |
| 66180 | pET15b+PCNA3 R112C | Expresses the R112C mutant of Sulfolobus solfataricus PCNA3 |
| 66181 | pET15b+PCNA3 T180C | Expresses the T180C mutant of Sulfolobus solfataricus PCNA3 |
| 66182 | pET15b+PCNA3 R112C/T180C | Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3 |