Electron donation to an archaeal cytochrome P450 is enhanced by PCNA-mediated selective complex formation with foreign redox proteins.
Suzuki R, Hirakawa H, Nagamune T
Biotechnol J. 2014 Dec;9(12):1573-81. doi: 10.1002/biot.201400007. Epub 2014 Jul 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 66131 | pET11a+CYP119 | Expresses the wild type of CYP119 |
| 66134 | pET11a+T214V | Expresses the T214V mutant of CYP119 |
| 66135 | pET11a+D77R/T214V | Expresses the D77R/T214V mutant of CYP119 |
| 66136 | pET11+PCNA3-CYP119 | Expresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3 |
| 66137 | pET11+PCNA3-CYP119(T214V) | Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3 |
| 66138 | pET11+PCNA3-CYP119(D77R/T214V) | Expresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3 |