Visualization of a functional Galpha q-green fluorescent protein fusion in living cells. Association with the plasma membrane is disrupted by mutational activation and by elimination of palmitoylation sites, but not be activation mediated by receptors or AlF4-.
Hughes TE, Zhang H, Logothetis DE, Berlot CH
J Biol Chem. 2001 Feb 9;276(6):4227-35. Epub 2000 Nov 13.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
66080 | G protein alpha-q-GFP | This G protein alpha-q construct contains internal insertions of GFP and the EE epitope |
66081 | G protein alpha-q-CFP | This G protein alpha-q construct contains internal insertions of CFP and the EE epitope |