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Single vector system for efficient N-myristoylation of recombinant proteins in E. coli.

Gluck JM, Hoffmann S, Koenig BW, Willbold D
PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
66078pETDuet-1∆HisTag_hNMT_NefSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli system
66079pETDuet-1∆HisTag_Nef_MCS2Single vector system expressing NEF allowing myristoylation of recombinant protein produce in Ecoli system

Antibodies from Article