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Quantifying cellular capacity identifies gene expression designs with reduced burden.

Ceroni F, Algar R, Stan GB, Ellis T
Nat Methods. 2015 May;12(5):415-8. doi: 10.1038/nmeth.3339. Epub 2015 Apr 6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
66073Burden Monitor lambda versionThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cells
66074Burden Monitor phi80 versionThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cell
66076pInt-tsThe pInt-ts plasmid [Haldimann2001] is the lambda helper plasmid necessary for the genome integration of the burden monitor lambda version
66077pAH123The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Antibodies from Article