Genetically encoded sensors of protein hydrodynamics and molecular proximity.
Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
65894 | 130_pSKB3-LUMP-P.leioghnati | Bacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime |
65972 | 140_pSKB3-GBD-LUMP | Bacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42 |
65973 | 213-petDuet1-GBD-LUMP--cdc42 | Dual expression of GBD-LUMP and cdc42 |
65975 | 234_pSKB3-Venus-LUMP | Bacterial expression of Venus-LUMP FRET pair |
65976 | 274_pSKB3-Venus-LUMP | Bacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor |