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Genetically encoded sensors of protein hydrodynamics and molecular proximity.

Hoepker AC, Wang A, Le Marois A, Suhling K, Yan Y, Marriott G
Proc Natl Acad Sci U S A. 2015 Apr 30. pii: 201424021. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65894130_pSKB3-LUMP-P.leioghnatiBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetime
65972140_pSKB3-GBD-LUMPBacterial expression of GBD-LUMP protein, a sensor for GTP-bound Cdc42
65973213-petDuet1-GBD-LUMP--cdc42Dual expression of GBD-LUMP and cdc42
65975234_pSKB3-Venus-LUMPBacterial expression of Venus-LUMP FRET pair
65976274_pSKB3-Venus-LUMPBacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensor

Antibodies from Article