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C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization.

Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. Epub 2015 Jan 20. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65843pcDNA3.2-C11orf83-V5Mammalian expression of C terminally V5-tagged C11orf83/UQCC3
65844pcDNA-Dest47- C11orf83-GFPMammalian expression of C terminally GFP-tagged C11orf83/UQCC3
65845pcDNA-Dest47- WTN23C11orf83-GFPMammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)
65846pcDNA-Dest47- R5A-K5A N23 C11orf83-GFPMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)
65847pcDNA-Dest47- R5D-K6D N23 C11orf83-GFPMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)
65848pDEST15-GST-C11orf83bacterial expression of GST (N-ter) - C11orf83/UQCC3
65849GST-Delta23C11orf83bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)
65850pcDNA3.2-R5A-K6A C11orf83-V5Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6A
65851pcDNA3.2-R5D-K6D C11orf83-V5Mammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6D

Antibodies from Article