Controlling gene expression with the Q repressible binary expression system in Caenorhabditis elegans.
Wei X, Potter CJ, Luo L, Shen K
Nat Methods. 2012 Mar 11;9(4):391-5. doi: 10.1038/nmeth.1929.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65832 | XW08 | Punc-4-QF-SL2-mcherry-3'UTR-unc-54 |
| 65833 | XW09 | Punc-4c-QS-SL2-mcherry3'UTR-unc-54 |
| 65834 | XW12 | Pquas-Δpes10-GFP-3'UTR-unc-54 |
| 65835 | XW17 | Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77) |
| 65836 | XW20 | Punc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry) |
| 65837 | XW21 | XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry) |
| 65838 | XW52 | XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method |
| 65839 | XW54 | Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method |
| 65840 | XW82 | MosSCI-QUAS::Δpes10::GFP |
| 65841 | XW83 | MosSCI-Pmig-13::QF::SL2::mCherry |
| 65842 | XW99 | MosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I. |