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Addgene

Assembly and specific recognition of k29- and k33-linked polyubiquitin.

Michel MA, Elliott PR, Swatek KN, Simicek M, Pruneda JN, Wagstaff JL, Freund SM, Komander D
Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
65437pOPINK-TRABID (NZF1, aa 1-33)Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli
65441pOPINB-OTUB1*Expresses activated OTUB1 in E. coli
66185pOPINK-TRABID (NZF1-3, aa 1-178)Expresses TRABID NZF1-3 (aa 1-178) in E. coli
66710pOPINS-AREL1Expresses AREL1 HECT domain in E. coli
66711pOPINS-UBE3CExpresses UBE3C HECT domain in E. coli
66712pOPINB-AMSH*Expresses activated AMSH in E. coli

Antibodies from Article