Tools for Anopheles gambiae Transgenesis.
Volohonsky G, Terenzi O, Soichot J, Naujoks DA, Nolan T, Windbichler N, Kapps D, Smidler AL, Vittu A, Costa G, Steinert S, Levashina EA, Blandin SA, Marois E
G3 (Bethesda). 2015 Apr 13. pii: g3.115.016808. doi: 10.1534/g3.115.016808.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62289 | pDSAG | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker |
| 62290 | pDSAT | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker |
| 62291 | pDSAY | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP marker |
| 62292 | pDSAR | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP marker |
| 62293 | pDSAP | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker |
| 62294 | pDSAYN | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, YFP-NLS marker |
| 62295 | pDSARN | docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker |
| 62296 | pattB-RfB2 | docking site transgenesis plasmid for Gateway Cloning and insect transgenesis |
| 62299 | pENTR R4-vas2-integrase-R3 | phageC31 integrase-expressing helper plasmid for Anopheles transgenesis, vasa promoter |
| 62301 | pENTR L1-vas2-Cre-L4 | Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter |
| 62302 | pDSAR-Cre-F2A-I-SceI | Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker |
| 62540 | pSKB- | Cloning vector to prepare modules flanked by appropriately designed BsaI sites for seamless assembly of multiple inserts by GoldenGate Cloning |