Plug-and-play genetic access to drosophila cell types using exchangeable exon cassettes.
Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, Potter CJ, Landgraf M, White BH
Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62891 | pT-GEM(0) | Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons |
| 62893 | pT-GEM(1) | Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons |
| 62894 | pT-GEM(2) | Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons |
| 62896 | pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0. |
| 62897 | pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1. |
| 62898 | pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2. |
| 62899 | pBS-KS-attB2-SA(0)-T2A-Gal4 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62900 | pBS-KS-attB2-SA(1)-T2A-Gal4 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62901 | pBS-KS-attB2-SA(2)-T2A-Gal4 | Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62902 | pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70 | Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62903 | pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70 | Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62904 | pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70 | Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62905 | pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70 | Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62908 | pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70 | Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62911 | pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70 | Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62912 | pBS-KS-attB2-SA(0)-T2A-p65AD-Hsp70 | Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62914 | pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70 | Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62915 | pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70 | Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62944 | pBS-KS-attB2-SA(0)-T2A-QF2-Hsp70 | Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62945 | pBS-KS-attB2-SA(1)-T2A-QF2-Hsp70 | Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62946 | pBS-KS-attB2-SA(2)-T2A-QF2-Hsp70 | Creating QF2 drivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62947 | pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70 | Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0 |
| 62948 | pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70 | Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1 |
| 62949 | pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70 | Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2 |
| 62951 | pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70 | Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP. |
| 62952 | pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70 | Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP. |
| 62953 | pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70 | Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP. |
| 62954 | pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70) | Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver. |
| 62955 | pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70) | Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver. |
| 62956 | pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70) | Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver. |
| 62957 | pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3 | Triplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver. |
| 78304 | pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70 | Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0 |
| 78306 | pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70 | Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1 |
| 78307 | pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70 | Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2 |