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A RhoC biosensor reveals differences in the activation kinetics of RhoA and RhoC in migrating cells.

Zawistowski JS, Sabouri-Ghomi M, Danuser G, Hahn KM, Hodgson L
PLoS One. 2013 Nov 5;8(11):e79877. doi: 10.1371/journal.pone.0079877. eCollection 2013. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
65071RhoC FLARE.sc mCer, mVenus - wtRhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC
65423RhoC FLARE.sc mCer, mVenus - DNRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N
65424RhoC FLARE.sc mCer, mVenus - CARhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Antibodies from Article