The interaction of cytoplasmic poly(A)-binding protein with eukaryotic initiation factor 4G suppresses nonsense-mediated mRNA decay.
Fatscher T, Boehm V, Weiche B, Gehring NH
RNA. 2014 Oct;20(10):1579-92. doi: 10.1261/rna.044933.114. Epub 2014 Aug 21.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65807 | pCI-MS2V5-PABPC1 | expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops. |
| 65808 | pCI-MS2V5-PABPC1 MLLEmut | expression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain. |
| 65809 | pCI-MS2V5-PABPC1 M161A/D165K | expression of PABPC1-MS2 fusion. Mutations abolish binding to eIF4G |
| 65810 | pCI-MS2V5-PABPC1 MLLEmut M161A/D165K | expression of PABPC1-MS2 fusion, double mutant. |
| 65811 | pCI-MS2V5-eRF3a F76a | expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1 |
| 65812 | pCI-MS2V5-eIF4G delN83 | expression of eIF4G-MS2 fusion. |