mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa.
Choi KH, Schweizer HP
Nat Protoc. 2006;1(1):153-61.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65022 | pUC18R6K-mini-Tn7T-Gm | GmR mini-Tn7 vector, R6K replicon |
| 65024 | pUC18T-mini-Tn7T-Tp | TpR mini-Tn7 vector, mobilizable |
| 65026 | pUC18-mini-Tn7T-Gm-LacZ10 | GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence. |
| 65027 | pUC18-mini-Tn7T-Gm-LacZ20 | GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence. |
| 65028 | pUC18-mini-Tn7T-Gm-LacZ30 | GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence. |
| 65030 | pUC18T-mini-Tn7T-Gm-ecfp | GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria |
| 65031 | pUC18T-mini-Tn7T-Gm-eyfp | GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria |
| 65032 | pUC18T-mini-Tn7T-Gm-dsRedExpress | GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria |
| 65033 | pUC18T-mini-Tn7T-Tp-gfpmut3 | TpR mini-Tn7 vector, mobilizable, for GFP tagging bacteria |
| 65034 | pUC18T-mini-Tn7T-Tp-ecfp | TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria |
| 65035 | pUC18T-mini-Tn7T-Tp-eyfp | TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria |
| 65036 | pUC18T-mini-Tn7T-Tp-dsRedExpress | TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria |
| 65037 | pUC18T-mini-Tn7T-Zeo-gfpmut3 | ZeoR mini-Tn7 vector, mobilizable, for GFP tagging bacteria |
| 65038 | pUC18T-mini-Tn7T-Zeo-ecfp | ZeoR mini-Tn7 vector, mobilizable, for CFP tagging bacteria |
| 65039 | pUC18T-mini-Tn7T-Zeo-eyfp | ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria |
| 65040 | pUC18T-mini-Tn7T-Zeo-dsRedExpress | ZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteria |