Noninvasive optical inhibition with a red-shifted microbial rhodopsin.
Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES
Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65012 | fck-Jaws-KGC-GFP-ER2 | Lentivirus-mediated expression of Jaws under the CaMKII promoter. |
| 65013 | fck-Jaws-GFP-ER2 | Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter. |
| 65014 | pAAV-hsyn-Jaws-KGC-GFP-ER2 | AAV-mediated expression of Jaws under the human synapsin promoter |
| 65015 | pAAV-CaMKII-Jaws-KGC-GFP-ER2 | AAV-mediated expression of Jaws under the CaMKII promoter |
| 65016 | pAAV-hsyn-Jaws-GFP-ER2 | AAV-mediated expression of Jaws-ER2 under the human synapsin promoter |
| 78174 | pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2] | AAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner. |
| 84445 | pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] | AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner. |
| 84446 | pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] | AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner. |
| 99233 | pAAV-CAG-Jaws-KGC-GFP-ER2 | AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter. Using bGH pA signal. |
| 108274 | pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2] | AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. |
| 111549 | 20XUAS IVS Jaws mCherry_tr | Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry |
| 111550 | 10XUAS IVS Jaws mCherry_tr | Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry |
| 111551 | 13XLexAop2 IVS Jaws mCherry_tr | Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry |
| 111552 | 20XUAS IVS Jaws mVenus_tr | Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus |
| 111553 | 10XUAS IVS Jaws mVenus_tr | Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus |
| 153534 | AAV-CaMKII-Jaws-KGC-tdTomato-ER2 | AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CaMKII promoter. |
| 153535 | AAV-Syn-Jaws-KGC-tdTomato-ER2 | AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter. |
| 153536 | pAAV-Syn-FLEX-rc [Jaws-KGC-tdTomato-ER2] | AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. |
| 153537 | pAAV-EF1α1.1-Jaws-KGC-GFP-ER2 | AAV-mediated expression of Jaws-GFP-ER2 under the EF1α promoter (1.1kb short version). |
| 153538 | pAAV-EF1α1.1-Jaws-KGC-tdTomato-ER2 | AAV-mediated expression of Jaws-tdTomato-ER2 under the EF1α promoter (1.1kb short version). |
| 195488 | AAV-CAG-FLPX-rc [Jaws-KGC-tdTomato-ER2] | AAV-mediated expression of Jaws-KGC-tdTomato-ER2 under the CAG promoter, in FLPX/reversed (Frt-dependent) manner. |
| 196140 | AAV-CAG-Cre | AAV-mediated expression of Cre under the CAG promoter . |