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Design of Ca2+-independent Staphylococcus aureus sortase A mutants.

Hirakawa H, Ishikawa S, Nagamune T
Biotechnol Bioeng. 2012 Dec;109(12):2955-61. doi: 10.1002/bit.24585. Epub 2012 Jul 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64973pTH1-SaSrtAExpresses the wild type of Staphylococcus aureus sortase A in E. coli
64978pTH1-SaSrtA-KExpresses the E105K mutant of Staphylococcus aureus sortase A in E. coli
64979pTH1-SaSrtA-AExpresses the E108A mutant of Staphylococcus aureus sortase A in E. coli
64980pTH1-SaSrtA-QExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli
64981pTH1-SaSrtA-KAExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli
64982pTH1-SaSrtA-KQExpresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli
64987pTH1-SpSrtAExpresses the wild type of Streptococcus pyogenes sortase A in E. coli

Antibodies from Article