Dynamic Visualization of mTORC1 Activity in Living Cells.
Zhou X, Clister TL, Lowry PR, Seldin MM, Wong GW, Zhang J
Cell Rep. 2015 Mar 11. pii: S2211-1247(15)00177-1. doi: 10.1016/j.celrep.2015.02.031.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64927 | pcDNA3-TORCAR | FRET-based biosensor for monitoring mTORC1 kinase activity. |
| 64928 | pcDNA3-TORCAR(T/A) | Negative-control mutant for TORCAR biosensor. |
| 64929 | pcDNA3-Lyso-TORCAR | Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane. |
| 64930 | pcDNA3-PM-TORCAR | Plasma membrane-targeted FRET biosensor for monitoring mTORC1 kinase activity. |
| 64931 | pcDNA3-TORCAR-NLS | Nuclear-targeted FRET biosensor for monitoring mTORC1 kinase activity. |
| 64932 | pcDNA3-AktAR2 | Enhanced FRET-based biosensor for monitoring Akt activity. |
| 64933 | pcDNA3-Lyso-AktAR2 | Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane. |