Ca -independent sortase-A exhibits high selective protein ligation activity in the cytoplasm of E. coli.
Hirakawa H, Ishikawa S, Nagamune T
Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
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PubMed
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64983 | pASKt15C+SrtA | Expresses the wild type of Staphylococcus aureus sortase A in E. coli |
| 64984 | pASKt15C+SrtA2 | Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli |
| 64985 | pASKt15C+SrtA5 | Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli |
| 64986 | pASKt15C+SrtA7 | Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli |
| 65017 | pASKt15C+SrtAwoH | Expresses the wild type of Staphylococcus aureus sortase A in E. coli |
| 65018 | pASKt15C+SrtA2woH | Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli |
| 65019 | pASKt15C+SrtA5woH | Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus |
| 65020 | pASKt15C+SrtA7woH | Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli |