Mechanism of suppression of chromosomal instability by DNA polymerase POLQ.
Yousefzadeh MJ, Wyatt DW, Takata K, Mu Y, Hensley SC, Tomida J, Bylund GO, Doublie S, Johansson E, Ramsden DA, McBride KM, Wood RD
PLoS Genet. 2014 Oct 2;10(10):e1004654. doi: 10.1371/journal.pgen.1004654. eCollection 2014 Oct.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64873 | pBluescript II SK--POLQ | cloning vector for human POLQ |
| 64874 | pCDH-EF1-FHC | lentiviral vector with Flag, HA tags |
| 64875 | pCDH-EF1-FHC-POLQ | lentiviral expression of human POLQ |
| 64876 | pCDH-EF1-FHC-POLQ-S1977P | lentiviral expression of human POLQ S1977P mutant (mimicks the chaos1 mutation) |
| 64877 | pCDH-EF1-FHC-POLQ-K121M | lentiviral expression of human POLQ K121M mutant (a mutation in the conserved ATP-binding site of the Walker A motif in the helicase-like domain) |
| 64878 | pCDH-EF1-FHC-POLQ-DY2230AA | lentiviral expression of human POLQ -DY2230AA mutant (a polymerase domain mutant) |
| 64880 | pCDH-EF1-FHC-POLQ-DM | lentiviral expression of human POLQ double mutant (in POL and HDL domains) |