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Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals by calcineurin.

Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
64728pcDNA3-CaNAR2FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.
64729pcDNA3-Cyto-CaNAR2Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
64730pcDNA3-PM-CaNAR2Plasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.
64731pcDNA3-Mito-CaNAR2Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.
64732pcDNA3-ER-CaNAR2Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of ER membrane.
64733pcDNA3-ER-AKAR4Endoplasmic reticulum (ER)-targeted FRET-based PKA activity reporter; targeted to cytosolic face of ER membrane.
64848pcDN3.1(+)-CaNARiFRET-based calcineurin (CaN) activation biosensor.
64849pcDNA3.1(+)-Cyto-CaNARiCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.
64850pcDNA3.1(+)-PM-CaNARiPlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.
64851pcDNA3.1(+)-Mito-CaNARiMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.
64852pcDNA3.1(+)-ER-CaNARiEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.
64853pcDNA3-Cyto-GCaMP3Cytosol-targeted version of the Ca2+ indicator GCaMP3.
64854pcDNA3-ER-GCaMP3Endoplasmic reticulum (ER)-targeted version of the Ca2+ indicator GCaMP3; targeted to cytosolic face of ER membrane.
64855pcDNA3.1(+)-ER-BSCaM2Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

Antibodies from Article