Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.

Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA
Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
84443	pSF4 CMV intron renilla TRICK CTE polyA	TRICK reporter mRNA
71244	pCasper4-g-osk-TRICK	oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.
71243	pHsp83-NLS-HA-2xPCP-2xGFP	Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.
71242	pHsp83-NLS-HA-2xMCP-2xTagRFP-T	Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.
64544	pSF4 CMV 5TOP intron renilla TRICK CTE polyA	5′ TOP TRICK reporter mRNA
64542	pmax pona 12xTRICK 24xMS2SL	TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.
64541	phage UBC NLS-HA-2XMCP-tagRFPt	Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP.
64540	phage ubc nls ha 2xmcp HALO	Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.
64539	phage ubc nls ha pcp gfp	Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.