Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.
Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer RH, Chao JA
Science. 2015 Mar 20;347(6228):1367-671. doi: 10.1126/science.aaa3380.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 64539 | phage ubc nls ha pcp gfp | Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP. |
| 64540 | phage ubc nls ha 2xmcp HALO | Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO. |
| 64541 | phage UBC NLS-HA-2XMCP-tagRFPt | Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to tagRFP. |
| 64542 | pmax pona 12xTRICK 24xMS2SL | TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops. |
| 64544 | pSF4 CMV 5TOP intron renilla TRICK CTE polyA | 5′ TOP TRICK reporter mRNA |
| 71242 | pHsp83-NLS-HA-2xMCP-2xTagRFP-T | Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T. |
| 71243 | pHsp83-NLS-HA-2xPCP-2xGFP | Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP. |
| 71244 | pCasper4-g-osk-TRICK | oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila. |
| 84443 | pSF4 CMV intron renilla TRICK CTE polyA | TRICK reporter mRNA |