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Addgene

Analyze Sequence Guide

To analyze a sequence, paste your sequence into the box, then click Submit. You will have the following options:

  • Enter a new sequence link: Start over
  • Map and Features tab:
      This link will show a map of your sequence, with features and restriction sites listed below the map. Only commonly used enzymes are displayed. Use the links at the bottom to show or hide features.
  • Sequence tab:
    • FASTA: View your sequence in FASTA format (not numbered).
    • GenBank: View your sequence in GenBank format (features listed, numbered).
    • Reverse Complement: View the reverse complement of your sequence.
  • BLAST tab:
    • Determine what your sequence encodes by using BLASTN to search for a DNA match or BLASTX to search for a protein match.
  • Align tab:
    • Compare your sequence to another sequence.
  • Digest tab:
    • View restriction sites in your sequence. View unique cutters, non-cutters, and expected cut sites.
  • Translate tab:
    • View open reading frames in your sequence with common tags highlighted. Also see a translation of your sequence in all reading frames.