Mark Styczynski Lab Materials

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Title	Authors	Publication
A Cell-Free Biosensor for Assessment of Hyperhomocysteinemia.	Piorino F, Johnson S, Styczynski MP	ACS Synth Biol. 2023 Aug 18;12(8):2487-2492. doi: 10.1021/acssynbio.3c00103. Epub 2023 Jul 17.
Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B(12) Biosensor.	McNerney MP, Piorino F, Michel CL, Styczynski MP	ACS Synth Biol. 2020 Feb 21;9(2):402-411. doi: 10.1021/acssynbio.9b00429. Epub 2020 Feb 13.
Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency.	Piorino F, Styczynski MP	ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
Point-of-care biomarker quantification enabled by sample-specific calibration.	McNerney MP, Zhang Y, Steppe P, Silverman AD, Jewett MC, Styczynski MP	Sci Adv. 2019 Sep 25;5(9):eaax4473. doi: 10.1126/sciadv.aax4473. eCollection 2019 Sep.
Styczynski Lab TLISA plasmids 2024	(unpublished)

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
	216324	pT7_zntR	Zinc-responsive activator	Bacterial Expression	Point-of-care biomarker quantification enabled by sample-specific calibration. Sci Adv. 2019 Sep 25;5(9):eaax4473. doi: 10.1126/sciadv.aax4473. eCollection 2019 Sep.
	216325	pzntA_LacZ	b-Galactosidase	Bacterial Expression	Point-of-care biomarker quantification enabled by sample-specific calibration. Sci Adv. 2019 Sep 25;5(9):eaax4473. doi: 10.1126/sciadv.aax4473. eCollection 2019 Sep.
	216326	pCF	Superfolder green fluorescent protein	Bacterial Expression	Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency. ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
	216327	pUlaR	L-Ascorbate-6-P-responsive transciptional repressor	Bacterial Expression	Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency. ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
	216328	pBad_UlaABC	UlaABC	Bacterial Expression	Harnessing Escherichia coli's Native Machinery for Detection of Vitamin C (Ascorbate) Deficiency. ACS Synth Biol. 2022 Nov 18;11(11):3592-3600. doi: 10.1021/acssynbio.2c00335. Epub 2022 Oct 27.
	216329	pMetR	MetR	Bacterial Expression	A Cell-Free Biosensor for Assessment of Hyperhomocysteinemia. ACS Synth Biol. 2023 Aug 18;12(8):2487-2492. doi: 10.1021/acssynbio.3c00103. Epub 2023 Jul 17.
	216334	pEutRwc	EutR, Superfolder green fluorescent protein	Bacterial Expression	Active Analyte Import Improves the Dynamic Range and Sensitivity of a Vitamin B(12) Biosensor. ACS Synth Biol. 2020 Feb 21;9(2):402-411. doi: 10.1021/acssynbio.9b00429. Epub 2020 Feb 13.
	216337	pLaG2-T7RNAPC	LaG2-T7RNAPC fusion	Bacterial Expression	Styczynski Lab TLISA plasmids 2024 (unpublished)
	216338	pT7RNAPNev-FSR22	T7RNAPN-FSR22 fusion	Bacterial Expression	Styczynski Lab TLISA plasmids 2024 (unpublished)