Lukas Kapitein Lab Materials

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Title	Authors	Publication
A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle.	Jansen KI, Iwanski MK, Burute M, Kapitein LC	J Cell Biol. 2023 May 1;222(5):e202106105. doi: 10.1083/jcb.202106105. Epub 2023 Mar 2.
An optimized toolbox for the optogenetic control of intracellular transport.	Nijenhuis W, van Grinsven MMP, Kapitein LC	J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
Bacterial expression plasmids for the purification of labelled DmKHC for motor-PAINT	(unpublished)
Differentiation between Oppositely Oriented Microtubules Controls Polarized Neuronal Transport.	Tas RP, Chazeau A, Cloin BMC, Lambers MLA, Hoogenraad CC, Kapitein LC	Neuron. 2017 Dec 20;96(6):1264-1271.e5. doi: 10.1016/j.neuron.2017.11.018. Epub 2017 Nov 30.
Direct observation of aggregate-triggered selective autophagy in human cells.	Janssen AFJ, Korsten G, Nijenhuis W, Katrukha EA, Kapitein LC	J Cell Sci. 2021 Oct 1;134(19). pii: 271972. doi: 10.1242/jcs.258824. Epub 2021 Oct 6.
Direct observation of motor protein stepping in living cells using MINFLUX.	Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J	Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9.
Imaging of KIF5B and Tubulin	(unpublished)
Local changes in microtubule network mobility instruct neuronal polarization and axon specification.	Burute M, Jansen KI, Mihajlovic M, Vermonden T, Kapitein LC	Sci Adv. 2022 Nov 4;8(44):eabo2343. doi: 10.1126/sciadv.abo2343. Epub 2022 Nov 4.
Nuclear poly-glutamine aggregates rupture the nuclear envelope and hinder its repair.	Korsten G, Osinga M, Pelle RA, Serweta AK, Hoogenberg B, Kampinga HH, Kapitein LC	J Cell Biol. 2024 Nov 4;223(11):e202307142. doi: 10.1083/jcb.202307142. Epub 2024 Aug 16.
Probing aggrephagy using chemically-induced protein aggregates.	Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, Kapitein LC	Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
20.41.586557.174649	174649	pB80-hKif5b(1-560)rigor-2xmNeongreen	hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)	Mammalian Expression	A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle. J Cell Biol. 2023 May 1;222(5):e202106105. doi: 10.1083/jcb.202106105. Epub 2023 Mar 2.
20.12.884514.111758	111758	pBA-mCherry-EGFP-PIM	2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP	Mammalian Expression	Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
20.0.616577.111759	111759	pGW1-mCherry-EGFP-PIM	2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP	Mammalian Expression	Probing aggrephagy using chemically-induced protein aggregates. Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
	174621	pB80-empty		Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174622	pB80-FKBP-mCherry-RAB11	FKBP-mCherry-RAB11 (RAB11A Synthetic, Human)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174623	pB80-iLID-mCherry-RAB11	iLID-mCherry-RAB11 (RAB11A Synthetic, Human)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174624	pB80-iLID-mCherry-RAB5	iLID-mCherry-RAB5 (RAB5A Synthetic, Human)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174625	pB80-LAMP1-mCherry-iLID	LAMP1-mCherry-iLID (LAMP1 Synthetic, Human)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174626	pB80-KIF1A(1-383)-GFP-SSPB(milli)	KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Mouse, Synthetic)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.
	174627	pB80-KIF1A(1-383)-GFP-SSPB(micro)	KIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)	Mammalian Expression	An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.