Kai Johnsson Lab Materials

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Title	Authors	Publication
A biosensor for measuring NAD(+) levels at the point of care.	Yu Q, Pourmandi N, Xue L, Gondrand C, Fabritz S, Bardy D, Patiny L, Katsyuba E, Auwerx J, Johnsson K	Nat Metab. 2019 Dec;1(12):1219-1225. doi: 10.1038/s42255-019-0151-7. Epub 2019 Dec 13.
A fluorescent sensor for GABA and synthetic GABA(B) receptor ligands.	Masharina A, Reymond L, Maurel D, Umezawa K, Johnsson K	J Am Chem Soc. 2012 Nov 21;134(46):19026-34. doi: 10.1021/ja306320s. Epub 2012 Nov 5.
A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range.	Xue L, Prifti E, Johnsson K	J Am Chem Soc. 2016 Apr 27;138(16):5258-61. doi: 10.1021/jacs.6b03034. Epub 2016 Apr 15.
AspSnFR: A genetically encoded biosensor for real-time monitoring of aspartate in live cells.	Hellweg L, Pfeifer M, Tarnawski M, Thing-Teoh S, Chang L, Bergner A, Kress J, Hiblot J, Wiedmer T, Superti-Furga G, Reinhardt J, Johnsson K, Leippe P	Cell Chem Biol. 2024 May 20:S2451-9456(24)00179-X. doi: 10.1016/j.chembiol.2024.05.002.
Bioluminescent Antibodies for Point-of-Care Diagnostics.	Xue L, Yu Q, Griss R, Schena A, Johnsson K	Angew Chem Int Ed Engl. 2017 Jun 12;56(25):7112-7116. doi: 10.1002/anie.201702403. Epub 2017 May 16.
Chemogenetic Control of Nanobodies.	Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K	Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7.
Engineered HaloTag variants for fluorescence lifetime multiplexing.	Frei MS, Tarnawski M, Roberti MJ, Koch B, Hiblot J, Johnsson K	Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x.
Environmentally Sensitive Color-Shifting Fluorophores for Bioimaging.	Wang L, Hiblot J, Popp C, Xue L, Johnsson K	Angew Chem Int Ed Engl. 2020 Dec 1;59(49):21880-21884. doi: 10.1002/anie.202008357. Epub 2020 Sep 28.
Evaluating Cellular Drug Uptake with Fluorescent Sensor Proteins.	Scarabelli S, Tan KT, Griss R, Hovius R, D'Alessandro PL, Vorherr T, Johnsson K	ACS Sens. 2017 Aug 25;2(8):1191-1197. doi: 10.1021/acssensors.7b00331. Epub 2017 Aug 2.
Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.	Haruki H, Gonzalez MR, Johnsson K	PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 2012 May 31.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
100.18.199819.136626	136626	pET51b(+)_EGFP_NbALFA	EGFP-NbALFA	Bacterial Expression	Chemogenetic Control of Nanobodies. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7.
50.33.564694.167266	167266	pET51b-His-TEV-HaloTag7	HaloTag7	Bacterial Expression	Kinetic and Structural Characterization of the Self-Labeling Protein Tags HaloTag7, SNAP-tag, and CLIP-tag. Biochemistry. 2021 Aug 24;60(33):2560-2575. doi: 10.1021/acs.biochem.1c00258. Epub 2021 Aug 2.
20.38.128950.175539	175539	pcDNA5/FRT/TO_[Cox8a]x2_HaloTag9	Cox8a-HaloTag9	Mammalian Expression	Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x.
20.28.592174.175544	175544	pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb	IgKchL_HA-HaloTag9-myc_PDGFRtmb	Mammalian Expression	Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x.
20.8.344916.175547	175547	pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9	H2B-HaloTag7 and Tomm20-HaloTag9	Mammalian Expression	Engineered HaloTag variants for fluorescence lifetime multiplexing. Nat Methods. 2021 Dec 16. pii: 10.1038/s41592-021-01341-x. doi: 10.1038/s41592-021-01341-x.
20.5.139482.113917	113917	pEBTet_NADP-Snifit-cyto	NADP-Snifit	Mammalian Expression	Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
20.4.772284.113919	113919	pEBTet_NADP-Snifit-mito	NADP-Snifit	Mammalian Expression	Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
20.2.979253.127233	127233	pCDNA3.1-V5-PTK2B	PTK2B	Mammalian Expression	Exploiting ligand-protein conjugates to monitor ligand-receptor interactions. PLoS One. 2012;7(5):e37598. doi: 10.1371/journal.pone.0037598. Epub 2012 May 31.
20.2.277717.136628	136628	pET51b(+)_ALFA-tag-SNAP	EGFP-NbALFA	Bacterial Expression	Chemogenetic Control of Nanobodies. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi: 10.1038/s41592-020-0746-7.
20.1.315174.113916	113916	pEBTet_NAD-Snifit-mito	NAD-Snifit	Mammalian Expression	Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.