Gerold Schmitt-Ulms Lab Materials

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Title	Authors	Publication
CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome.	Mehrabian M, Brethour D, MacIsaac S, Kim JK, Gunawardana CG, Wang H, Schmitt-Ulms G	PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014.
Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies.	Wang X, Friesen E, Muller I, Lemieux M, Dukart R, Maia IB, Kalia S, Schmitt-Ulms G	Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
20.1.985007.132389	132389	AAVS1 CAG rtTA3 TauWT 2N4R-EGFP	human TauWT 2N4R-EGFP (MAPT Human, Synthetic)	Mammalian Expression, Cre/Lox, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.
	61856	MLM3636-Prnp-CDS-Neg	PrP guiding sequence (Prnp Mouse)	Mammalian Expression, CRISPR	CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome. PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014.
	61857	MLM3636-Prnp-CDS-Pos	PrP guiding sequence (Prnp Mouse)	Mammalian Expression, CRISPR	CRISPR-Cas9-Based Knockout of the Prion Protein and Its Effect on the Proteome. PLoS One. 2014 Dec 9;9(12):e114594. doi: 10.1371/journal.pone.0114594. eCollection 2014.
	132388	AAVS1 KanR foundation lox casette	G418 resistance	Mammalian Expression, Cre/Lox, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.
	132393	AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP	human TauP301L 2N4R-EGFP (MAPT Human, Synthetic)	Mammalian Expression, Cre/Lox, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.
	132394	AAVS1 intron 1 gRNA as2	gRNA targeting AAVS1 intron 1 (PPP1R12C Human)	Mammalian Expression, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.
	132395	AAVS1 intron 1 gRNA s3	gRNA targeting AAVS1 intron 1 (PPP1R12C Human)	Mammalian Expression, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.
	132396	AAVS1 intron 1 gRNA 27	gRNA targeting AAVS1 intron 1 (PPP1R12C Human)	Mammalian Expression, CRISPR	Rapid Generation of Human Neuronal Cell Models Enabling Inducible Expression of Proteins-of-interest for Functional Studies. Bio Protoc. 2020 May 5;10(9):e3615. doi: 10.21769/BioProtoc.3615. eCollection 2020 May 5.