David Savage Lab Materials

The David Savage Lab has deposited materials at Addgene for distribution to the research community. Addgene is a nonprofit plasmid repository dedicated to improving life science research.

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Title	Authors	Publication
Conserved and repetitive motifs in an intrinsically disordered protein drive ⍺-carboxysome assembly.	Turnsek JB, Oltrogge LM, Savage DF	J Biol Chem. 2024 Jul 4;300(8):107532. doi: 10.1016/j.jbc.2024.107532.
CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification.	Oakes BL, Fellmann C, Rishi H, Taylor KL, Ren SM, Nadler DC, Yokoo R, Arkin AP, Doudna JA, Savage DF	Cell. 2019 Jan 10;176(1-2):254-267.e16. doi: 10.1016/j.cell.2018.11.052.
DABs are inorganic carbon pumps found throughout prokaryotic phyla.	Desmarais JJ, Flamholz AI, Blikstad C, Dugan EJ, Laughlin TG, Oltrogge LM, Chen AW, Wetmore K, Diamond S, Wang JY, Savage DF	Nat Microbiol. 2019 Aug 12. pii: 10.1038/s41564-019-0520-8. doi: 10.1038/s41564-019-0520-8.
Functional reconstitution of a bacterial CO2 concentrating mechanism in E. coli.	Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D	Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin.	Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, East-Seletsky A, Williams ER, Savage DF	Biochemistry. 2016 Jun 21;55(24):3461-8. doi: 10.1021/acs.biochem.6b00294. Epub 2016 Jun 7.
iLOV Recombineering Target Plasmid	(unpublished)
Modularity of a carbon-fixing protein organelle.	Bonacci W, Teng PK, Afonso B, Niederholtmeyer H, Grob P, Silver PA, Savage DF	Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):478-83. doi: 10.1073/pnas.1108557109. Epub 2011 Dec 19.
Multivalent interactions between CsoS2 and Rubisco mediate alpha-carboxysome formation.	Oltrogge LM, Chaijarasphong T, Chen AW, Bolin ER, Marqusee S, Savage DF	Nat Struct Mol Biol. 2020 Mar;27(3):281-287. doi: 10.1038/s41594-020-0387-7. Epub 2020 Mar 2.
Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch.	Oakes BL, Nadler DC, Flamholz A, Fellmann C, Staahl BT, Doudna JA, Savage DF	Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
Rapid and Programmable Protein Mutagenesis Using Plasmid Recombineering.	Higgins SA, Ouonkap SVY, Savage DF	ACS Synth Biol. 2017 Jul 24. doi: 10.1021/acssynbio.7b00112.

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	ID	Plasmid	Gene/Insert	Vector Type	Publication
50.6.925240.79769	79769	pUCKanR-Mu-BsaI	Mu-BsaI		Rapid construction of metabolite biosensors using domain-insertion profiling. Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
20.4.419830.79770	79770	pATT-Dest		Synthetic Biology	Rapid construction of metabolite biosensors using domain-insertion profiling. Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
20.3.627363.79784	79784	pTKEI-Dest		Bacterial Expression	Rapid construction of metabolite biosensors using domain-insertion profiling. Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
20.1.312525.74494	74494	pBLO1811_arC9_noNLS_human	arC9	Mammalian Expression, CRISPR	Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
20.0.372907.79754	79754	pTKEI-Tre-C04	Tre-C04	Bacterial Expression	Rapid construction of metabolite biosensors using domain-insertion profiling. Nat Commun. 2016 Jul 29;7:12266. doi: 10.1038/ncomms12266.
	52064	pHnCB	CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B	Bacterial Expression	Modularity of a carbon-fixing protein organelle. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):478-83. doi: 10.1073/pnas.1108557109. Epub 2011 Dec 19.
	52065	pHnCBS1D	CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D	Bacterial Expression	Modularity of a carbon-fixing protein organelle. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):478-83. doi: 10.1073/pnas.1108557109. Epub 2011 Dec 19.
	74490	pBLO1811_Cas9_noNLS_human	Cas9	Mammalian Expression	Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
	74491	pBLO1808_arC9_2xNLS_human	arC9	Mammalian Expression	Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.
	74493	pBLO1806_Cas9_2xNLS_human	Cas9	Mammalian Expression	Profiling of engineering hotspots identifies an allosteric CRISPR-Cas9 switch. Nat Biotechnol. 2016 May 2. doi: 10.1038/nbt.3528.