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Addgene

pACT2-Rcl1
(Plasmid #99992)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99992 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACT2
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rcl1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Contains amino acids 1-217 of Rcl1
  • Entrez Gene
    RCL1 (a.k.a. YOL010W)
  • Tag / Fusion Protein
    • Y2H activation domain (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Compared to the best match NCBI reference sequence EIW07424.1, Addgene NGS results found A24T and R207S

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACT2-Rcl1 was a gift from Susan Baserga (Addgene plasmid # 99992 ; http://n2t.net/addgene:99992 ; RRID:Addgene_99992)

  • For your References section:

    The SSU processome interactome in Saccharomyces cerevisiae reveals potential new protein subcomplexes. Vincent NG, Charette JM, Baserga SJ. RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117. 10.1261/rna.062927.117 PubMed 29054886
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