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Addgene

pcDNA3.1-TFEBS211A-MYC (#805)
(Plasmid #99957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TFEB
  • Species
    H. sapiens (human)
  • Mutation
    S211A
  • Entrez Gene
    TFEB (a.k.a. ALPHATFEB, BHLHE35, TCFEB)
  • Tags / Fusion Proteins
    • Myc (C terminal on backbone)
    • 6xHIs (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-TFEBS211A-MYC (#805) was a gift from James Brugarolas (Addgene plasmid # 99957 ; http://n2t.net/addgene:99957 ; RRID:Addgene_99957)

  • For your References section:

    Multistep regulation of TFEB by MTORC1. Vega-Rubin-de-Celis S, Pena-Llopis S, Konda M, Brugarolas J. Autophagy. 2017 Mar 4;13(3):464-472. doi: 10.1080/15548627.2016.1271514. Epub 2017 Jan 5. 10.1080/15548627.2016.1271514 PubMed 28055300
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