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Addgene

pcDNA3-HA-REDD1 101-232 (#183)
(Plasmid #99923)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99923 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    REDD1
  • Species
    H. sapiens (human)
  • Mutation
    N-terminal deletion; encodes AA 101-232
  • Entrez Gene
    DDIT4 (a.k.a. Dig2, REDD-1, REDD1)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-HA-REDD1 101-232 (#183) was a gift from James Brugarolas (Addgene plasmid # 99923 ; http://n2t.net/addgene:99923 ; RRID:Addgene_99923)

  • For your References section:

    Structural analysis and functional implications of the negative mTORC1 regulator REDD1. Vega-Rubin-de-Celis S, Abdallah Z, Kinch L, Grishin NV, Brugarolas J, Zhang X. Biochemistry. 2010 Mar 23;49(11):2491-501. doi: 10.1021/bi902135e. 10.1021/bi902135e PubMed 20166753
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