psgRNA-ccdB
(Plasmid
#99915)
-
Purpose(Empty Backbone) Basic cloning vector for the expression of imaging-based optimization sgRNA
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 99915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA3.1(+)
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
psgRNA-ccdB was a gift from Yujie Sun (Addgene plasmid # 99915 ; http://n2t.net/addgene:99915 ; RRID:Addgene_99915) -
For your References section:
Multiplexed sgRNA Expression Allows Versatile Single Nonrepetitive DNA Labeling and Endogenous Gene Regulation. Shao S, Chang L, Sun Y, Hou Y, Fan X, Sun Y. ACS Synth Biol. 2017 Sep 7. doi: 10.1021/acssynbio.7b00268. 10.1021/acssynbio.7b00268 PubMed 28849913