Skip to main content
Addgene

pET21a-GFP-AURKA_K162M-mCherry
(Plasmid #99881)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99881 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    MerckMillipore
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AURKA
  • Species
    H. sapiens (human)
  • Mutation
    K162M (kinase-dead)
  • Entrez Gene
    AURKA (a.k.a. AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7)
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Same construct as pET21-GFP-AURKA-mCherry obtained by directed mutagenesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21a-GFP-AURKA_K162M-mCherry was a gift from Marc Tramier (Addgene plasmid # 99881 ; http://n2t.net/addgene:99881 ; RRID:Addgene_99881)

  • For your References section:

    A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells. Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M. Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674. 10.1038/ncomms12674 PubMed 27624869
Related items:
Commonly requested with: