pAuroraA-GFP-AURKA_K162M-mCherry
(Plasmid #99880)

• Purpose: Expression of GFP-AURKA K162M-mCherry in cells
• Depositing Lab: Marc Tramier
• Publication: Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: CMV promoter was substituted by the AURKA minimal promoter sequence (CTTCCGG) into the AseI/BspEI restriction sites
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AURKA
• Species: H. sapiens (human)
• Mutation: K162M (kinase-dead)
• Entrez Gene: AURKA (a.k.a. AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7)
• Promoter: AURKA minimal
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • mCherry (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP-C

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Same construct as pAuroraA-GFP-AURKA-mCherry obtained by directed mutagenesis