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Addgene

pCETN1-IRFP670
(Plasmid #99879)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99879 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piRFP670-N1
  • Backbone manufacturer
    Addgene
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CETN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CETN1 (a.k.a. CEN1, CETN)
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP670 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCETN1-IRFP670 was a gift from Marc Tramier (Addgene plasmid # 99879 ; http://n2t.net/addgene:99879 ; RRID:Addgene_99879)

  • For your References section:

    A FRET biosensor reveals spatiotemporal activation and functions of aurora kinase A in living cells. Bertolin G, Sizaire F, Herbomel G, Reboutier D, Prigent C, Tramier M. Nat Commun. 2016 Sep 14;7:12674. doi: 10.1038/ncomms12674. 10.1038/ncomms12674 PubMed 27624869
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