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Addgene

PB-UniSAM
(Plasmid #99866)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99866 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Piggy Bac
  • Backbone size w/o insert (bp) 3961
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; dCAS9-SAM activation
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    UniSAM-mCherry + U6-gRNA2.0
  • Alt name
    Cas9VP64-MS2p65HSF1-mCherry + gRNA(2xMS2Loops)
  • Insert Size (bp)
    8388
  • Mutation
    BbsI sites in CDS were ablated by consensus mutation codon optimised for H.sapiens
  • Promoter EF1a

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer EXT_PB_RV--GGCACATATCAATATTATGCTCTCG
  • 3′ sequencing primer EXT_PB_FW--GCAAAGCGATGACGAGCTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-UniSAM was a gift from Lesley Forrester (Addgene plasmid # 99866 ; http://n2t.net/addgene:99866 ; RRID:Addgene_99866)

  • For your References section:

    An all-in-one UniSam vector system for efficient gene activation. Fidanza A, Lopez-Yrigoyen M, Romano N, Jones R, Taylor AH, Forrester LM. Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.1038/s41598-017-06468-6. 10.1038/s41598-017-06468-6 PubMed 28743878
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