AAV-KrasG12S/sgKras/Cre
(Plasmid #99853)

• Purpose: Expresses Cre-recombinase, a barcoded HDR template to introduce a G12S mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.
• Depositing Lab: Monte Winslow
• Publication: Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi: 10.1038/s41467-017-01519-y.

Backbone

• Vector backbone: 388-MCS AAV
• Backbone size w/o insert (bp): 5958
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kras
• gRNA/shRNA sequence: GACTGAGTATAAACTTGTGG
• Species: M. musculus (mouse)
• Insert Size (bp): 2014
• Mutation: AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutations (GACTGAGTATAAACTTGTGGTGG>GACTGAGTATAAACTAGTAGTCG), G12S mutation (GGT>AGT), Barcode = (AGGCAAGAGCGCCTTGACGATA>TGGCAAGTCCGCCCTAACGATC), BsiWI site (CGTGCG>CGTACG)
• Entrez Gene: Kras (a.k.a. K-Ras, K-Ras 2, K-ras, Ki-ras, Kras-2, Kras2, c-K-ras, c-Ki-ras, p21B, ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI, AvrII (not destroyed)
• 3′ cloning site: BsiWI, XhoI (not destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

https://benchling.com/s/seq-atBNRmhI5tEHkQKLBbHp