pHL-FcHis
(Plasmid #99846)

• Purpose: mammalian expression with secretive signal and FC tag
• Depositing Lab: Edith Yvonne Jones
• Publication: Aricescu et al Acta Crystallogr D Biol Crystallogr. 2006 Oct;62(Pt 10):1243-50. Epub 2006 Sep 19.

Backbone

• Vector backbone: pCAG.I-SceI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ephrin b2
• Species: H. sapiens (human)
• Entrez Gene: EPHB2 (a.k.a. BDPLT22, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5)
• Tag / Fusion Protein:
  • Fc_His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: KpnI (unknown if destroyed)
• 5′ sequencing primer: CTCTAGAGCCTCTGCTAAC
• 3′ sequencing primer: GCTGGGCACGGTGGGCATG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry