env8 selectivity (b')
(Plasmid
#99837)
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PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99837 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRR5
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameenv8 selectivity (b')
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MutationA20G, A68G, G-C pairs in P1 and P3
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Tag
/ Fusion Protein
- mNeonGreen (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Selectivity switch from MeCbl to AdoCbl
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
env8 selectivity (b') was a gift from Robert Batey (Addgene plasmid # 99837 ; http://n2t.net/addgene:99837 ; RRID:Addgene_99837) -
For your References section:
Cobalamin Riboswitches Exhibit a Broad Range of Ability to Discriminate between Methylcobalamin and Adenosylcobalamin. Polaski JT, Webster SM, Johnson JE Jr, Batey RT. J Biol Chem. 2017 May 8. pii: jbc.M117.787176. doi: 10.1074/jbc.M117.787176. 10.1074/jbc.M117.787176 PubMed 28483920
