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Addgene

env8 reverse J1/13
(Plasmid #99831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRR5
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    env8 reverse J1/13
  • Mutation
    J1/13 sequence is reversed
  • Tag / Fusion Protein
    • GFPuv (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    env8 reverse J1/13 was a gift from Robert Batey (Addgene plasmid # 99831 ; http://n2t.net/addgene:99831 ; RRID:Addgene_99831)

  • For your References section:

    Mechanistic Insights into Cofactor-Dependent Coupling of RNA Folding and mRNA Transcription/Translation by a Cobalamin Riboswitch. Polaski JT, Holmstrom ED, Nesbitt DJ, Batey RT. Cell Rep. 2016 May 3;15(5):1100-10. doi: 10.1016/j.celrep.2016.03.087. Epub 2016 Apr 21. 10.1016/j.celrep.2016.03.087 PubMed 27117410
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