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Addgene

CDC25-1
(Plasmid #99763)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99763 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CDC25-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene Note: These plasmids were generated for in vitro transcription and may not be suitable for overexpression studies. The inserts were amplified from cDNA and may differ from NCBI reference sequences.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CDC25-1 was a gift from Phillip Newmark (Addgene plasmid # 99763 ; http://n2t.net/addgene:99763 ; RRID:Addgene_99763)

  • For your References section:

    A premeiotic function for boule in the planarian Schmidtea mediterranea. Iyer H, Issigonis M, Sharma PP, Extavour CG, Newmark PA. Proc Natl Acad Sci U S A. 2016 Jun 21;113(25):E3509-18. doi: 10.1073/pnas.1521341113. Epub 2016 Jun 2. 10.1073/pnas.1521341113 PubMed 27330085
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