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Addgene

iChr2-Notch Mosaic (SO250)
(Plasmid #99752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript KS
  • Total vector size (bp) 27430
  • Vector type
    Mammalian Expression, Mouse Targeting, Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    HIs-H2B-Cherry, H2B-EGFP-V5, HA-H2B-Cerulean, DN-Maml1, NICD-PEST
  • Species
    M. musculus (mouse), Synthetic

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iChr2-Notch Mosaic (SO250) was a gift from Rui Benedito (Addgene plasmid # 99752 ; http://n2t.net/addgene:99752 ; RRID:Addgene_99752)

  • For your References section:

    Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R. Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031. 10.1016/j.cell.2017.07.031 PubMed 28802047
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