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pcDNA4/TO h-Stx3S-2xMyc/His
(Plasmid #99744)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99744 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4
  • Backbone manufacturer
    Thermo Fisher
  • Backbone size w/o insert (bp) 5075
  • Total vector size (bp) 5909
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Stx3S
  • Alt name
    Stx3 isoform 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    834
  • GenBank ID
    NM_001178040.1
  • Entrez Gene
    STX3 (a.k.a. DIAR12, MVID2, RDMVID, STX3A)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc-myc-his (C terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer acgcaaatgggcggtaggcgtg
  • 3′ sequencing primer tagaaggcacagtcgaggctg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4/TO h-Stx3S-2xMyc/His was a gift from Thomas Weimbs (Addgene plasmid # 99744 ; http://n2t.net/addgene:99744 ; RRID:Addgene_99744)

  • For your References section:

    Soluble syntaxin 3 functions as a transcriptional regulator. Giovannone AJ, Winterstein C, Bhattaram P, Reales E, Low SH, Baggs JE, Xu M, Lalli MA, Hogenesch JB, Weimbs T. J Biol Chem. 2018 Apr 13;293(15):5478-5491. doi: 10.1074/jbc.RA117.000874. Epub 2018 Feb 23. 10.1074/jbc.RA117.000874 PubMed 29475951
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