pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP
(Plasmid
#99731)
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Purposeoverexpression of PDGFRa and CopGFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99731 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH-CMV-MCS-EF1-nlsCopGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePdgfra
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SpeciesM. musculus (mouse)
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Insert Size (bp)3340
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Entrez GenePdgfra (a.k.a. CD140a, Pdgfr-2)
- Promoter CMV
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDH-CMV-PDGFRa-3xFlag-EF1-nlsCopGFP was a gift from Mengsheng Qiu (Addgene plasmid # 99731 ; http://n2t.net/addgene:99731 ; RRID:Addgene_99731) -
For your References section:
Tmeff2 is expressed in differentiating oligodendrocytes but dispensable for their differentiation in vivo. Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M. Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1. 10.1038/s41598-017-00407-1 [pii] PubMed 28336932