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pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP
(Plasmid #99730)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1-copGFP
  • Vector type
    Mammalian Expression
  • Selectable markers
    copGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmeff2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Tmeff2 (a.k.a. 4832418D20Rik, 7630402F16Rik, TR-2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xMyc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-CMV-Tmeff2-2xMyc-EF1-copGFP was a gift from Mengsheng Qiu (Addgene plasmid # 99730 ; http://n2t.net/addgene:99730 ; RRID:Addgene_99730)

  • For your References section:

    Tmeff2 is expressed in differentiating oligodendrocytes but dispensable for their differentiation in vivo. Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M. Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1. 10.1038/s41598-017-00407-1 [pii] PubMed 28336932
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