Skip to main content
Addgene

pT7T3D-PacI-Tmeff2
(Plasmid #99728)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 99728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT7T3D-PacI
  • Vector type
    in situ probe

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tmeff2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    767
  • Entrez Gene
    Tmeff2 (a.k.a. 4832418D20Rik, 7630402F16Rik, TR-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid contains bp 474-1125 of Tmeff2 for synthesis of in situ RNA probe.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT7T3D-PacI-Tmeff2 was a gift from Mengsheng Qiu (Addgene plasmid # 99728 ; http://n2t.net/addgene:99728 ; RRID:Addgene_99728)

  • For your References section:

    Tmeff2 is expressed in differentiating oligodendrocytes but dispensable for their differentiation in vivo. Huang H, Teng P, Mei R, Yang A, Zhang Z, Zhao X, Qiu M. Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1038/s41598-017-00407-1. 10.1038/s41598-017-00407-1 [pii] PubMed 28336932
Related items: