pAAV-SCP1-dSa-VPR mini.
(Plasmid #99685)

• Purpose: Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter
• Depositing Lab: George Church
• Publication: Vora et al bioRxiv 2018. 298620

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Backbone

• Vector backbone: pAAV (pUC f1)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Species: Synthetic
• Mutation: dead Cas9
• Tag / Fusion Protein:
  • VPR mini (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/298620 for bioRxiv preprint.