pAAV-dSa-VPR
(Plasmid #99651)

• Purpose: Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
• Depositing Lab: George Church
• Publication: Church Lab CRISPR plasmids 2017 (unpublished)

Backbone

• Vector backbone: pAAV (pUC f1)
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Species: Synthetic
• Mutation: dead Cas9
• Tag / Fusion Protein:
  • VP64-p65-RTA activator (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • Map_1_AAV_NLS-dS.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry