px330 Rosa26 Gu1 (LH416)
(Plasmid
#99629)
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PurposePlasmid used to target the wildtype Rosa26 allele with Cas9
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepx330_U6_Chimeric
- Total vector size (bp) 8378
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
px330 Rosa26 Gu1 (LH416) was a gift from Rui Benedito (Addgene plasmid # 99629 ; http://n2t.net/addgene:99629 ; RRID:Addgene_99629) -
For your References section:
Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R. Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031. 10.1016/j.cell.2017.07.031 PubMed 28802047
