Triple ORF FRT ifgMosaic Donor (IG135)
(Plasmid
#99626)
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PurposeTo clone the 3 ORFs for FlpO dependent generation of ifgMosaics
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC18
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Vector typeMouse Targeting, Cre/Lox, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Triple ORF FRT ifgMosaic Donor (IG135) was a gift from Rui Benedito (Addgene plasmid # 99626 ; http://n2t.net/addgene:99626 ; RRID:Addgene_99626) -
For your References section:
Dual ifgMosaic: A Versatile Method for Multispectral and Combinatorial Mosaic Gene-Function Analysis. Pontes-Quero S, Heredia L, Casquero-Garcia V, Fernandez-Chacon M, Luo W, Hermoso A, Bansal M, Garcia-Gonzalez I, Sanchez-Munoz MS, Perea JR, Galiana-Simal A, Rodriguez-Arabaolaza I, Del Olmo-Cabrera S, Rocha SF, Criado-Rodriguez LM, Giovinazzo G, Benedito R. Cell. 2017 Aug 10;170(4):800-814.e18. doi: 10.1016/j.cell.2017.07.031. 10.1016/j.cell.2017.07.031 PubMed 28802047