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PurposeLentivirus expressing puromycin resistance gene and TEV protease
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 99610 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbone3rd gen lentiviral vector
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructionsStbl2 or NEB Stable can also be used
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namePuromycin resistance (PAC gene)
Gene/Insert 2
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Gene/Insert nameTEV protease
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-CMV-puro-2A-TEVp was a gift from Marco Tripodi (Addgene plasmid # 99610 ; http://n2t.net/addgene:99610 ; RRID:Addgene_99610) -
For your References section:
Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus. Ciabatti E, Gonzalez-Rueda A, Mariotti L, Morgese F, Tripodi M. Cell. 2017 Jul 1. pii: S0092-8674(17)30697-9. doi: 10.1016/j.cell.2017.06.014. 10.1016/j.cell.2017.06.014 PubMed 28689641